Detection of High Methylation of p15(INK4B) and p16(INK4A) Genes in Multiple Myeloma
- Author:
Hongtao FAN
1
,
2
,
3
;
Xiuzhi GUO
;
Qiong WU
;
Tao ZHOU
;
Guangxiao TAN
;
Luya WANG
;
Xueli ZHANG
;
Gengxin LUO
;
Minhua XU
Author Information
1. Departimet of Hematology, Medical College, Jinan University, Guangzhou 510632, China
2. Howard University, USA Beijing Anzhen Hospital, Beijing 100029, China
3. Department of Hematology, The First Affiliated Hospital of Medical College, Jinan University, Guangzhou 510632, China.
- Publication Type:Journal Article
- From:
Journal of Experimental Hematology
2000;8(4):271-274
- CountryChina
- Language:Chinese
-
Abstract:
To study the action, characteristics and expression of high methylation of p15(INK4B) and p16(INK4A) genes in multiple myeloma (MM), the sensitive methylation specific PCR method was employed to detect the hypermethylation of p15(INK4B) and p16(INK4A) in 24 patients with MM. Results showed that the methylation incidence of p15(INK4B) and p16(INK4A) genes were 70.8% (17/24) and 58.3% (14/24) in the MM patients, with the products of 148 bp and 150 bp fragments, respectively. The methylation of p15(INK4B) and p16(INK4A) genes were simultaneously happened in MM patients of plasmocytoma type with two cases at II phase and two cases at III phase. The simultaneous non-methylation of p15(INK4B) and p16(INK4A) genes were founded in five cases of MM patients, all of the tumor cells were of small plasmocyte type with mature differention. Conclusion suggested that there were high incidence of methylation of p15(INK4B) and p16(INK4A) genes in patients with MM. Hypermethylation can be detected in the early stage of disease, which was associated with its progress. It indicated a bad prognosis when methylation happended simultaneously in the two genes. Methylation of p15(INK4B) and p16(INK4A) genes may be related to the pathogeny of MM.
