Standardization and Quality Control in Flow Cytometric Enumeration of CD34(+) cells
- Author:
Yanrong LIU
1
;
Shanshan CHEN
;
Hong YU
Author Information
1. Institute of Hematology, Peking University, Beijing 100044, China.
- Publication Type:Journal Article
- From:
Journal of Experimental Hematology
2000;8(4):302-306
- CountryChina
- Language:Chinese
-
Abstract:
The enumeration of CD34(+) cells by flow cytometry is commonly employed to assess hematopoietic stem/progenitor cells in cord blood, peripheral blood and apheresis products. Interlaboratory variation of CD34(+) cells enumeration is exceedingly large. Factors contributing to those variation, currently established flow cytometry protocols and comparison between these protocols were reviewed. CD34(+) cells in 45 cord blood samples, 12 normal bone marrows and 4 apheresis products were also enumerated in our laboratory by using ProCUNT kit, and results showed that the ProCUNT is highly standardizaed and could assist in reducing interlaboratory variation of CD34(+) cells.
