Development of Acute Promyelocytic Leukemia in PML-RARalpha Transgenic Mice
- Author:
Xiuqin MENG
1
,
2
;
Guoxiang CHENG
;
Wenjun CAO
;
Jiang ZHU
;
Jianquan CHEN
;
Zhu CHEN
;
Saijuan CHEN
Author Information
1. Shanghai Institute of Hematology, Affiliated Ruijin Hospital, Shanghai Second Medical University, Shanghai 200025, China
2. Shanghai Railway Center Hospital, Shanghai 200072, China.
- Publication Type:Journal Article
- From:
Journal of Experimental Hematology
2000;8(2):104-109
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the leukemogenic potential of PML-RARalpha fusion protein in vivo, hCG-PML-RARalpha transgene was constructed using molecular cloning technique and hCG-PML-RARalpha transgenic mice were generated. The genotype and phenotype of hCG-PML-RARalpha transgenic mice were analyzed by PCR, RT-PCR, morphology of peripheral blood and bone marrow cells, and pathological examination of spleen, liver and bone marrow. As a result, acute promyelocytic leukemia was developed in 3 hCG-PML-RARalpha transgenic mice in 1 - 5 months. The results demonstrated that PML-RARalpha fusion protein plays a crucial role in leukemogenesis.
