Expension In Vitro of T Cells from Cord Blood CD34(+) Cells Stimulated with SCF and IL-2
- Author:
Yuanlin LIU
1
;
Yongjun SUI
;
Shuangxi ZHANG
;
Zikuan GUO
;
Ying WU
;
Ning MAO
Author Information
1. Beijing Institute of Basic Medical Sciences, Beijing 100850, China.
- Publication Type:Journal Article
- From:
Journal of Experimental Hematology
2000;8(1):48-51
- CountryChina
- Language:Chinese
-
Abstract:
The generation of large quantities of novel human T cell clones ex vivo would make a wide range of gene-and immuno-therapies for tumor and AIDS possibly. Although it is well established that T cells are derived from CD34(+) cells, the involvement of thymic fragments from either human or murine fetus makes the in vitro T cell perliferation very cumbersome. In this report, cord blood mononuclear cells were used as accessory cells to support the differentiation of CD34(+) cells into naive T cells stimulated with SCF and IL-2. CD4(+) and CD8(+) T cells, under the cultural conditions, were continuously produced in vitro at least over a period of 3 weeks and their ratios changed gradually. CD4/CD8 double positive T cells and RAG-2 gene were existed, and RAG-2 gene, reponsible for TCR rearrangement, was expressed during the cell proliferation. Our study presents a simple culture system in vitro to acquire large quantities of naive T cell clones.
