Expression of CXCR4 and its effect on the biological behavior of multiple myeloma cells.
- Author:
Cai-Xia LI
1
;
De-Pei WU
;
Yue-Dan WANG
;
Ge-Hua YU
;
Ji-Ming LIU
;
Yu-Mei ZHUANG
;
Chang-Shuo YIN
;
Xue-Guang ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Aged; Cell Movement; Chemokine CXCL12; Chemokines, CXC; biosynthesis; Female; Humans; Intercellular Adhesion Molecule-1; biosynthesis; Male; Middle Aged; Monocytes; metabolism; pathology; Multiple Myeloma; metabolism; pathology; Receptors, CXCR4; biosynthesis; physiology; Tumor Cells, Cultured; Up-Regulation
- From: Chinese Journal of Hematology 2003;24(3):122-125
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of stromal cell derived factor-1alpha(SDF-1alpha) expression and its receptor CXCR4 on the biological behavior of multiple myeloma (MM) cells and on the expression of soluble intercellular adhesion molecule 1 (ICAM-1).
METHODSFACS analysis was used to study the expression of ICAM-1 (CD(54)) and CXCR4 on the surface of MM cells. Chemotaxis assay through transwell bore polycaronate and ELISA assay were employed to monitor the soluble ICAM-1 level.
RESULTS(1) Fresh MM cells expressed variable levels of functional CXCR4 [(50.4 +/- 27.3)%], which was correlated with the in vitro ability of transwell migration of MM cells [(23.6 +/- 17.2)%, P < 0.01]. (2) SDF-1alpha could up-regulate the expression of ICAM-1 on MM cells. Furthermore, the serum level of sICAM-1 was correlated with the expression of CXCR4 on MM cells.
CONCLUSIONSDF-1alpha/CXCR4 plays an important role on the biological behavior of MM cells via mediating the effect of adhesion molecules.
