Overexpression of S3 ribosomal protein gene is involved in drug resistance in K562/DOX cells.
- Author:
Ning-xi ZHU
1
;
Shu ZHENG
;
Rong-zhen XU
;
Rong-xi YU
Author Information
- Publication Type:Journal Article
- MeSH: Antibiotics, Antineoplastic; pharmacology; DNA, Antisense; genetics; DNA, Complementary; genetics; Doxorubicin; pharmacology; Drug Resistance, Neoplasm; genetics; Gene Expression; Humans; K562 Cells; Leukemia; genetics; pathology; Plasmids; genetics; Ribosomal Proteins; biosynthesis; genetics; Transfection
- From: Chinese Journal of Hematology 2003;24(3):141-143
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of overexpression of S3 ribosomal protein (S3rp) gene on the resistance of leukemia cell to antitumor drugs.
METHODSBoth sense and antisense cDNA recombinants of S3rp gene were constructed with pcDNA3.1 expression vector. Subsequently, the sense S3rp cDNA recombinant was transfected into K562 cells while the antisense one into K562/DOX cells (a multidrug resistant cell line). In addition, empty pcDNA3.1 vector was transfected into the corresponding cells as negative controls. The chemosensitivity of cells was evaluated by MTT assay.
RESULTSSense S3rp cDNA transfected K562 cells were 5.8 times more resistant to doxorubicin than control cells did, whereas antisense S3rp cDNA transfected K562/DOX cells were 3.2 times less resistant to doxorubicin than control cells did.
CONCLUSIONOverexpression of S3rp gene plays an important role in the development of drug resistance in K562/DOX cells.
