Study of murine hematopoietic stem/progenitor cell mobilized by recombinant human interleukin 11 combination with granulocyte-colony stimulating factor.
- Author:
Fan-yi MENG
1
;
Zu-jun JIANG
;
Zheng-shan YI
;
Dan XU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Drug Synergism; Female; Granulocyte Colony-Stimulating Factor; pharmacology; Hematopoietic Stem Cell Mobilization; methods; Hematopoietic Stem Cells; cytology; drug effects; Humans; Interleukin-11; pharmacology; Male; Mice; Mice, Inbred C57BL; Recombinant Proteins; pharmacology
- From: Chinese Journal of Hematology 2003;24(5):225-227
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the recombinant human interleukin 11 (rhIL-11) in combination with granulocyte-colony stimulating factor (rhG-CSF) for mobilizing peripheral blood stem/progenitor cells in C57BL/6 mice.
METHODSrhIL-11,250 micro g x kg(-1) x d(-1) per mouse alone or in combination with rhG-CSF 250 micro g x kg(-1) x d(-1) per mouse was administered to C57BL/6 mice from day 1 to 7. The changes of peripheral white blood cell count (WBC), platelet counts (BPC) and hematopoietic stem/progenitor cells yields were observed.
RESULTSThe results showed that rhIL-11 alone or in combination with rhG-CSF resulted in increase in absolute numbers of WBC, BPC, CD(34)(+) cells, and CFU-GM, CFU-E, CFU-MK yields in peripheral blood more than those of control (P < 0.001). The yields of CFU-MK was significantly more than that of rhG-CSF group (P < 0.001).
CONCLUSIONrhIL-11 alone or in combination with G-CSF could significantly mobilize hematopoietic stem/progenitor cells from bone marrow into peripheral blood.
