Analysis of CD117 expression on leukemia cells.
- Author:
Hong-qiang SHEN
1
;
Yong-min TANG
;
Shi-long YANG
;
Bo-qin QIAN
;
Hua SONG
;
Shu-wen SHI
;
Wei-qun XU
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Adult; Child; Child, Preschool; Diagnosis, Differential; Female; Flow Cytometry; Humans; Infant; Leukemia, Lymphoid; diagnosis; metabolism; Leukemia, Myeloid; diagnosis; metabolism; Male; Middle Aged; Proto-Oncogene Proteins c-kit; analysis; biosynthesis
- From: Chinese Journal of Hematology 2003;24(5):228-230
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the expression of CD(117) on different types of leukemia and its significance.
METHODSCD(117) expression was analysed by three-color flow cytometry with CD(45)/SSC gating strategy.
RESULTSCD(117) was expressed in 68% of acute myeloid leukemia (AML) and 80% of chronic myeloid leukemia in blast crisis (CML-BC) cases, but in only 2% of acute lymphoblastic leukemia cases. CD(117) was negative in CML in chronic phase and chronic lymphocytic leukemia. Cases in AML, CD(117) was expressed in 72% of M(0)/M(1), 88% of M(2), 50% of M(4), 75% of M(5a), 100% of M(6) and 100% of M(7) cases but was less expressed in M(3) (39%) and M(5b) (29%) cases. Expression of CD(117) was more frequent than CD(34) and HLA-DR in M(3) and some M(2) cases. There was a significant inverse relationship between CD(117) and CD(14) expression in AML.
CONCLUSIONSAnalysis of CD(117) expression may be of help in distinguishing myeloid from lymphoid leukemia and leukemia clone from normal cells.
