Expression of survivin in cord blood CD34(+) stem/progenitor cells and its significance.
- Author:
Yan-ping MA
1
;
Ping ZOU
;
Juan XIAO
;
Shi-ang HUANG
Author Information
- Publication Type:Journal Article
- MeSH: Antigens, CD34; blood; Cells, Cultured; Fetal Blood; cytology; Hematopoietic Cell Growth Factors; pharmacology; Hematopoietic Stem Cells; drug effects; metabolism; Humans; Inhibitor of Apoptosis Proteins; Microtubule-Associated Proteins; biosynthesis; genetics; physiology; Neoplasm Proteins; biosynthesis; genetics; physiology; RNA, Messenger; genetics; Reverse Transcriptase Polymerase Chain Reaction
- From: Chinese Journal of Hematology 2003;24(5):238-240
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the expression and significance of survivin protein and mRNA in cord blood (CB) CD(34)(+) stem/progenitor cells.
METHODSSurvivin level in CB CD(34)(+) cells was assessed by immuno-histochemistry and reverse transcription polymerase chain reaction (RT-PCR).
RESULTSSurvivin expressed in fresh CB CD(34)(+) cells, its protein level was 9.4 +/- 1.2, faint signal of the mRNA transcript band was detected. In the in vitro culture of the cells, hematopoietic growth factors could upregulate survivin expression, especially the combination of SCF, FL and Tpo. After 3 days culture, its protein level was 25.2 +/- 5.3, and mRNA transcript increased significantly. Survivin expression dropped down 24 hours after withdraw of these cytokines.
CONCLUSIONSurvivin is not a specific anti-apoptotic protein of cancer, and also expressed in normal hematopoietic stem/progenitor cells. It plays important regulating roles in the process of hematopoiesis.
