Transfection of chk1/2 antisense oligonucleotide to HL-60 cell line increases the apoptotic sensitivity to irradiation.
- Author:
Yi TANG
1
;
Wen-li LIU
;
Jian-feng ZHOU
;
Li-ping WAN
;
Qing-lei GAO
;
Jian-hong WU
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; genetics; radiation effects; Cell Cycle; radiation effects; Checkpoint Kinase 1; Checkpoint Kinase 2; HL-60 Cells; Humans; Oligonucleotides, Antisense; genetics; Protein Kinases; genetics; Protein-Serine-Threonine Kinases; genetics; Radiation Tolerance; Transfection
- From: Chinese Journal of Hematology 2003;24(5):253-255
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo block signal transduction of cell cycle checkpoints by antisense blocking of chk1/2 gene to increase the radiation sensitivity of HL-60 cell line.
METHODTo transfect the HL-60 cell with chk1/2 antisense and sense chain alone and in combination, expose the cells to irradiation at 24 h after the transfection, the chk1 protein change was assayed by Western blot and the cell cycles and annexin V apoptosis rates by FCM.
RESULTSThe irradiated apoptosis sensitivity was increased by antisense blocking of chk1 gene in HL-60 cell line, the apoptotic rate was 26.31% being significantly higher than that of the sense blocking (10.34%) (P < 0.05), Furthermore, the G(2)/M phase blocking phenomenon decreased and a synergic effect was observed in antisense blocking both the chk1 and chk2 genes.
CONCLUSIONAntisense blocking of chk1/chk2 could increase the apoptotic sensitivity to irradiation.
