Detection of 29 types of fusion gene in leukemia by multiplex RT-PCR.
- Author:
Zhi-gang LI
1
;
Min-yuan WU
;
Wei ZHAO
;
Bin LI
;
Jing YANG
;
Ping ZHU
;
Ya-mei HU
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Child; Child, Preschool; Female; Humans; Infant; Leukemia; genetics; Male; Oncogene Proteins, Fusion; genetics; Reverse Transcriptase Polymerase Chain Reaction; methods; Translocation, Genetic
- From: Chinese Journal of Hematology 2003;24(5):256-258
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo analyze the fusion genes derived from 29 types of chromosome structural aberrations in children with leukemia.
METHODSBone marrow samples from 191 children with leukemia were analyzed with a novel multiplex nested RT-PCR.
RESULTSOf the 191 leukemic samples, 86 (45.0%) carried 14 types of fusion genes including SIL/TAL1, MLL/AF1q, E2A/PBX1, MLL/AF6, AML1/ETO, MLL/AF9, TEL/ABL, BCR/ABL, MLL/AF10, dupMLL, MLL/ENL, TEL/AML1, PML/RARalpha and CBFbeta/MYH11. The activation of oncogene HOX11 was detected in 31 cases, with or without other chromosome aberrations in 15 (7.8%) and 16 cases (8.4%), respectively.
CONCLUSIONThis multiplex nested RT-PCR reaction could screen 29 types of chromosome structural aberrations at the same time. It may be helpful for the detection of minimal residual diseases after chemotherapy and bone marrow transplantation.
