Effect of T3 on the expression of transferrin receptor and ferritin in K562 cells and its possible mechanism.
- Author:
Mi ZHOU
1
;
Qing-kui LIAO
;
Feng-yi LI
;
Qiang LI
;
Chun-hua LUO
;
Ju GAO
;
Cang-song JIA
;
Chong-li YANG
Author Information
- Publication Type:Journal Article
- MeSH: Ferritins; biosynthesis; drug effects; genetics; Flow Cytometry; Gene Expression Regulation, Leukemic; drug effects; Humans; K562 Cells; RNA, Messenger; genetics; Radioimmunoassay; Receptors, Transferrin; biosynthesis; drug effects; genetics; Reverse Transcriptase Polymerase Chain Reaction; Triiodothyronine; pharmacology
- From: Chinese Journal of Hematology 2003;24(4):181-184
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the effect of T(3) on the expression of transferrin receptor (TfR) and ferritin (Fn) in K562 cells and its possible mechanism.
METHODSFlow cytometry was used for the detection of TfR expression, radioimmunoassay for Fn expression, RNA/protein band shift assay for the binding activity of iron regulatory protein (IRP) and iron responsive elements (IRE), and RT-PCR for TfR and Fn mRNA levels.
RESULTSDifferent concentration of T(3) significantly increased Fn expression of K562 cells, especially at 100 nmol/L and 200 nmol/L (p < 0.05). However, T(3) had no effect on TfR expression. T(3) decreased the binding activity between IRP and IRE, particularly at concentration of 50 nmol/L. Different concentration of T(3) increased Fn-H mRNA level at different time point while it had no effect on TfR mRNA level.
CONCLUSIONT(3) increased Fn expression of K562 cells through the possible mechanisms of either the post-transcriptional regulation or transcriptional modulation.
