Effects of bcr/abl fusion gene on expression of beta1 integrin and L-selectin in mouse chronic myeloid leukemia cells.
- Author:
Wei-liang WANG
1
;
Ti SHEN
;
Yu-rong HUI
;
Xi-chun GU
;
Rong-sheng LI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Line, Tumor; Fusion Proteins, bcr-abl; genetics; Gene Expression Regulation, Leukemic; Genes, Retinoblastoma; genetics; Integrin beta1; genetics; L-Selectin; genetics; Leukemia, Myelogenous, Chronic, BCR-ABL Positive; genetics; Mice; RNA, Messenger; genetics; Transfection
- From: Chinese Journal of Hematology 2003;24(7):337-339
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVESTo explore the effects of p210 bcr/abl fusion gene on expression of beta1 integrin and L-selectin mRNAs in mouse chronic myeloid leukemia (CML) cells.
METHODSComparisons of beta1 integrin and L-selectin mRNA levels among p210 bcr/abl negative, p210 bcr/abl positive, and p210 bcr/abl-Rb-C-Box positive cells were undertaken by quantity RT-PCR.
RESULTSIn p210 bcr/abl positive cells, L-selectin mRNA level was decreased, but beta1 integrin mRNA expression had no change as compared to those in p210 bcr/abl negative cells. When inhibition of bcr-abl tyrosine kinase activity by Rb-C-Box, the L-selectin mRNA expression restored to normal (similar to p210 bcr/abl negative cells).
CONCLUSIONp210 bcr/abl oncoprotein inhibits expression of L-selectin mRNA, but not of beta1 integrin mRNA.
