HLA-A genotyping by oligonucleotide arrays.
- Author:
Shou-wang HU
1
;
Fan ZHANG
;
Hui WANG
;
Yong-yao GENG
;
Sheng-qi WANG
Author Information
- Publication Type:Journal Article
- MeSH: Genotype; HLA-A Antigens; genetics; Humans; Oligonucleotide Array Sequence Analysis; methods; Oligonucleotide Probes; Sensitivity and Specificity
- From: Chinese Journal of Hematology 2003;24(7):340-343
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate HLA genotyping by oligonucleotide arrays.
METHODUnsymmetrical PCR was used to amplify HLA-A gene exon 2, 3. The PCR products were used as templates for hybridization. The oligonucleotide probes were spotted on glass to make microarrays. High signal and specific probes were selected. The effects of the length and location of probes on hybridization signal were studied. A set of computer software was designed for scanning and genotype differentiation.
RESULTThe genotypes of 30 samples analyzed by microarray showed concordance to that by SBT and PCR-SSP.
CONCLUSIONHLA-A genotyping by oligonucleotide array is a good method with advantage of high speed, low cost and high flux.
