Survivin antisense RNA enhances taxol-induced apoptosis in leukemia cell line HL-60.
- Author:
Xiao-juan WANG
1
;
Guo-yi DAI
;
Li-min CAO
;
Hui-fen ZHU
;
Yue ZHANG
;
Jing-fang SHAO
;
Jing YANG
;
Guan-xin SHEN
Author Information
- Publication Type:Journal Article
- MeSH: Antineoplastic Agents, Phytogenic; pharmacology; Apoptosis; drug effects; Drug Synergism; HL-60 Cells; Humans; Inhibitor of Apoptosis Proteins; Microtubule-Associated Proteins; genetics; Neoplasm Proteins; genetics; Paclitaxel; pharmacology; RNA, Antisense; genetics; pharmacology; Transfection
- From: Chinese Journal of Hematology 2003;24(7):351-354
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the effects of survivin antisense RNA on taxol-induced apoptosis in leukemia cell line HL-60.
METHODSA survivin antisense eukaryotic vector pcDNA3-SVVas was transferred into HL-60 cells by electroporation. The live fraction was determined by trypan blue dye exclusion assay. Cell counting and MTT assay were performed to evaluate the sensibility of the transfected cells to taxol. Apoptosis was detected by DNA gel electrophoresis and nuclear staining.
RESULTSTwo positive cell clones, HL-60 SVVas and HL-60 neo were obtained. Compared to HL-60 and HL-60 neo cells, HL-60 SVVas cells growth was significantly reduced (P < 0.05). By MTT assay, the IC(50) of taxol to HL-60 SVVas, HL-60 neo and HL-60 cells were (14.4 +/- 1.87) ng/ml, (31.9 +/- 6.38) ng/ml and (32.0 +/- 3.52) ng/ml, respectively, the difference was significant by statistic analysis (P < 0.01). Agarose gel electrophoresis of genomic DNA from HL-60 SVVas showed typical DNA ladder, but DNA from HL-60 neo and HL-60 did not. Nuclei become condense in HL-60 SVVas cells.
CONCLUSIONSurvivin antisense RNA could enhance taxol-induced apoptosis in leukemia cell line HL-60. This may lay an experimental foundation for further research of gene therapy in leukemia.
