Expression of nm23 genes in acute myeloid leukemia and its clinical significance.
- Author:
Qing-xiang MENG
1
;
Rong JIANG
;
Bao-qing YANG
;
Hong-yu ZHANG
;
Jin LIU
;
Li-ping PANG
;
Jun WANG
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Female; Gene Expression; Humans; Leukemia, Myeloid, Acute; genetics; pathology; Male; Middle Aged; NM23 Nucleoside Diphosphate Kinases; Nucleoside-Diphosphate Kinase; genetics; RNA, Messenger; genetics; Reverse Transcriptase Polymerase Chain Reaction
- From: Chinese Journal of Hematology 2003;24(7):369-371
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore nm23 gene mRNA expression and its clinical significance in acute leukemias (AML).
METHODSThe levels of nm23-H1 and nm23-H2 transcripts in 22 patients with acute myeloid leukemia (AML), 9 AML in complete remission (AML-CR), 12 acute lymphoblastic leukemia (ALL) and 4 chronic myeloid leukemia in chronic phase (CML-CP) were assayed by reverse transcriptase-polymerase chain reaction (RT-PCR).
RESULTSThe expression of nm23-H1 in AL especially in AML-M4 and AML-M5 was significantly higher than that in normal blood cells. An analysis of correlation between nm23 expression and clinicopathological parameters showed that increased nm23-H1 mRNA levels were associated with some poor-prognostic factors such as extramedullary infiltration, high white blood cell count (WBC), high lactate dehydrogenase (LDH) activity and high CD(7) expression, while inversely correlated with t(8; 21) and t(15; 17) which had a good-prognostic effect. The expression of nm23-H1 in AML patients in CR was significantly decreased compared with those untreated.
CONCLUSIONnm23-H1 was overexpressed in AL, especially in AML-M4 and AML-M5. High expression of nm23-H1 may be a poor prognostic factor.
