Expression of costimulatory molecules on peripheral blood lymphocytes of patients with idiopathic thrombocytopenic purpura.
- Author:
Yan-xia ZHAO
1
;
Qing-ping GAO
;
You-hua CHENG
;
Xu-bin AO
;
Lun-hua CHEN
;
Nian-lan YANG
;
Qiong-yu WANG
;
Li-yun SHEN
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Adult; Antigens, CD; blood; Blood Platelets; immunology; Female; Humans; Immunoglobulin G; blood; Lymphocyte Activation; Lymphocytes; chemistry; Male; Middle Aged; Purpura, Thrombocytopenic, Idiopathic; blood
- From: Chinese Journal of Hematology 2003;24(9):474-476
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the expressions of costimulators on peripheral T and B lymphocytes in patients with idiopathic thrombocytopenic purpura (ITP).
METHODSThe expression of B7-CD(28) and CD(40) of peripheral lymphocytes was measured by flow cytometry in 21 ITP patients and 9 normal subjects. The expression of PAIgG was measured by ELISA method.
RESULTSThe expression of CD(4)(+)CD(28)(+) was lower in ITP patients than in normal controls, but the expression of CD(86)(+) and CD(86)(+)CD(19)(+) was higher in ITP patients than in normal controls, while the expression of CD(80)(+), CD(40)(+), CD(28)(+), CD(19)(+)CD(86)(+), CD(19)(+)CD(40)(+), CD(4)(+)CD(28)(+)/CD(4)(+), CD(19)(+)CD(80)(+)/CD(19)(+) and CD(19)(+)CD(40)(+)/CD(19)(+) in ITP patients was normal. The PAIgG level was higher in 16 patients with a mean of (184.62 +/- 38.00) ng/10(7) plt. A positive correlation was found between PAIgG and CD(19)(+)CD(86)(+)/CD(19)(+) expression.
CONCLUSIONThere is no deficiency in expression of CD(28) on CD(4)(+) T lymphocytes in ITP patients. The change of CD(86) expression on B lymphocytes is possibly involved in pathophysiology of ITP, which may provide a theoretical instruction for ITP patients immunological therapy.
