Study on autoimmune thrombocytopenic purpura related antibody.
- Author:
Lu LU
1
;
Ming HOU
;
Yan SHI
;
Ping QIN
;
Jun PENG
;
Yuan-yuan ZHU
;
Mao-hong ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Adult; Autoantibodies; blood; Blood Platelets; immunology; Female; Humans; Male; Middle Aged; Platelet Glycoprotein GPIIb-IIIa Complex; immunology; Platelet Glycoprotein GPIb-IX Complex; immunology; Purpura, Thrombocytopenic, Idiopathic; immunology
- From: Chinese Journal of Hematology 2003;24(9):477-479
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate a specific and sensitive assay for the diagnosis of autoimmune thrombocytopenic purpura (AITP).
METHODSGlycoprotein specific autoantibodies in platelet eluate and plasma were detected by a modified monoclonal antibody immobilization of platelet antigens assay (MAIPA).
RESULTSThe overall positive rate of specific autoantibodies against platelet GPIIb/IIIa and GPIb/IX in plasma was 38.89% and in eluated platelet membrane was 68.52%. The difference between them was significant (corrected chi(2) = 19.39, P < 0.005). The proportion of positive MAIPA results between primary AITP and secondary AITP was not significantly different. There was a significant inverse correlation between antibody level and platelet count.
CONCLUSIONDetection of eluated GP-specific autoantibodies by MAIPA is highly specific and much more sensitive as compared with the measurement of their plasma counterparts in the diagnosing and therapeutic monitoring of AITP.
