Dynamic changes of cytokines in G-CSF mobilized peripheral blood.
- Author:
Cai-xia LI
1
;
De-pei WU
;
Wei-rong CHANG
;
Hua-ting ZHU
;
Jian-nong CEN
;
Xue-guang ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Adult; Enzyme-Linked Immunosorbent Assay; Female; Granulocyte Colony-Stimulating Factor; therapeutic use; Hematopoietic Stem Cell Mobilization; methods; Humans; Intercellular Adhesion Molecule-1; blood; Interleukin-8; blood; Male; Middle Aged; Treatment Outcome; Vascular Cell Adhesion Molecule-1; blood
- From: Chinese Journal of Hematology 2003;24(8):398-401
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the level and significance of interleukin-8 (IL-8), soluble intercellular adhesion molecule (sICAM-1) and soluble vascular cell adhesion molecule-1 (sVCAM-1) in patients peripheral blood (PB) during mobilization for peripheral blood stem cells harvesting.
METHODSThe levels of IL-8, sICAM-1 and sVCAM-1 in patients were dynamically assayed by ELISA during the mobilization procedure and the number of CD(34)(+) cell, white blood cell (WBC) and platelet (BPC) by flow cytometric analysis and hematometry respectively. Colony formation was assayed by using semisolid methycellulose culture.
RESULTSThere was a significant increase in plasma levels of IL-8 and both adhesion molecules [IL-8 (247.4 +/- 84.2) microg/L (P < 0.01); sICAM-1 (530.3 +/- 286.1) microg/L (P = 0.002 7); sVCAM-1 (575.3 +/- 350.4) microg/L (P = 0.001 3)] during the mobilization process; furthermore, IL-8 and sVCAM-1 concentration in the patient's plasma was paralleled to the numbers of CD(34)(+) cell, CFU-GM, WBC and BPC (P < 0.001).
CONCLUSIONThe levels of IL-8, sICAM-1 and sVCAM-1 in the patient's plasma were correlated to the PB number of CD(34)(+) cells, CFU-GM, WBC and BPC during the mobilization process. It suggested that analysis of IL-8, and sVCAM-1 dynamic changes may serve as markers for CD(34)(+) cells.
