Detection of cytomegalovirus infection by polymerase chain reaction in hematopoietic stem cell transplantation recipients.
- Author:
Lan-ping XU
1
;
Xiao-jun HUANG
;
Nai-lan GUO
;
Han-yun REN
;
Yao-chen ZHANG
;
Dao-pei LU
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Adult; Child; Child, Preschool; Cytomegalovirus; genetics; isolation & purification; Cytomegalovirus Infections; diagnosis; etiology; DNA, Viral; blood; urine; Female; Hematopoietic Stem Cell Transplantation; adverse effects; Humans; Male; Middle Aged; Polymerase Chain Reaction; methods; Retrospective Studies; Sensitivity and Specificity; Transplantation, Homologous; adverse effects
- From: Chinese Journal of Hematology 2003;24(8):407-409
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo evaluate the detection of cytomegalovirus (CMV) by polymerase chain reaction (PCR) for predicting the development of CMV disease.
METHODSOne hundred and thirty one allo-HSCT patients performed in the past 2 years were analyzed retrospectively. PCR-CMV was used to monitor CMV viremia and vireuria once a week after transplantation.
RESULTSIn the dynamic detection, CMV viremia was positive for at least one chance in 89 patients, vireuria did in 99 patients. Thirty-seven patients developed CMV disease with an accumulative incidence of 32.5%. The incidence of CMV disease was 15.6% in plasma CMV-PCR negative group, 31.3% in positive once group, and 47.3% in positive over twice group. There was significant difference among the three groups (P = 0.0126). The incidence of CMV disease was 24.8% in urine CMV-PCR negative group, 43.5% in positive once group, and 33.0% in positive over twice group, being no significant difference among them (P = 0.845). On analysis, viremia could predict the development of CMV disease: the PPV (positive predictive value) is 40.5%, NPV (negative predictive value) is 84.4%, sensitivity is 75.0%, and specificity is 69.2%.
CONCLUSIONSDetected by CMV-PCR, MCV viremia may predict the development of CMV disease, but MCV vireuria cannot.