Identification of differentially expressed genes in Lin-CD34- and Lin-CD34+ cells.
- Author:
Dong-mei WANG
1
;
Li YANG
;
Liang LI
;
Kai FENG
;
Ci-xian BAI
;
Lin CHEN
;
Xue-tao PEI
Author Information
- Publication Type:Journal Article
- MeSH: Antigens, CD34; metabolism; Cloning, Molecular; Gene Expression Profiling; Gene Library; Hematopoietic Stem Cells; cytology; metabolism; Humans; In Vitro Techniques; Nucleic Acid Hybridization
- From: Chinese Journal of Hematology 2003;24(8):423-425
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo identify genes that differentially expressed in Lin(-)CD(34)(-) and Lin(-)CD(34)(+) cells.
METHODSWith Lin(-)CD(34)(-) cells as tester and Lin(-)CD(34)(+) cells as driver, cDNA subtractive library for Lin(-)CD(34)(-) cells was constructed using suppression subtractive hybridization technique. Part of clones in the library were sequenced and the homologue analysis was conducted against the DNA database in GenBank.
RESULTS593 clones containing an average of 300 - 500 bp insert were identified. Of them, 53 randomly selected ESTs were sequenced. Homologue analysis revealed that 37 ESTs represented 10 known genes, and the other 16 ESTs represented 4 novel sequences.
CONCLUSIONPart of specifically expressed genes in Lin(-)CD(34)(-) cells were identified, which maybe related to Lin(-)CD(34)(-) cells' specific characteristics.
