Inhibition of tumor angiogenesis in nude mice by adenovirus-mediated PF4 p17-70 cDNA transfection.
- Author:
Li-hua WU
1
;
Guo-li SONG
;
Shi-yong DIAO
;
Ying-lin CAI
;
Yan-han LI
;
Shang-zhu LI
;
Ren-chi YANG
;
Zhong-chao HAN
Author Information
- Publication Type:Journal Article
- MeSH: Adenoviridae; genetics; Animals; Cell Proliferation; Endothelial Cells; cytology; Female; Genetic Therapy; methods; Genetic Vectors; Humans; Mice; Mice, Inbred BALB C; Mice, Nude; Neoplasms, Experimental; pathology; therapy; Neovascularization, Pathologic; therapy; Platelet Factor 4; genetics; Transfection; Umbilical Veins; cytology
- From: Chinese Journal of Hematology 2003;24(8):426-429
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the in vivo effect of modified platelet factor 4 (PF4)-p17-70 cDNA on tumor angiogenesis in nude mice.
METHODSThe p17-70 cDNA was cloned into the AdEasy system to transfect packing cell line 293 and produce viral particles encoding p17-70cDNA (Ad p17-70). The integration of p17-70 cDNA was confirmed by RT-PCR and the P17-40 peptide Western blot. The biological activity of purified recombinant adenovirus was determined by umbilical veinal endothelial cell proliferation assay in vitro and in vivo tumor angiogenesis suppression of nude mice bearing human head and neck carcinoma.
RESULTSp17-70 significantly inhibited in vitro proliferation of endothelial cells being 58% lower than that of empty vector and reduced tumor volume in vivo. The tumor mass was (0.086 +/- 0.054) g, (0.171 +/- 0.076) g and (0.195 +/- 0.067) g, the tumor volume was (16.7 +/- 5.2) mm(3), (36.5 +/- 23.7) mm(3) and (41.5 +/- 12.2) mm(3) in p17-70 cDNA transfected group, empty vector group and PBS group, respectively. Immunohistochemical staining demonstrated a decreased number of blood vessels in the tumors.
CONCLUSIONP17-70 peptide mediated by adenoviral vector could inhibit the endothelial proliferation in vitro and the tumor growth in vivo.
