Detection of IgH-MMSET fusion gene in multiple myeloma patients and its significance.
- Author:
Hua JIANG
1
;
Jian HOU
;
Yu-bao CHEN
;
Si-qi DING
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Aged; Base Sequence; Chromosomes, Human, Pair 14; Chromosomes, Human, Pair 4; Female; Humans; Male; Middle Aged; Molecular Sequence Data; Multiple Myeloma; genetics; Oncogene Proteins, Fusion; genetics; Protein-Tyrosine Kinases; Receptor, Fibroblast Growth Factor, Type 3; Receptors, Fibroblast Growth Factor; genetics; Reverse Transcriptase Polymerase Chain Reaction; Translocation, Genetic
- From: Chinese Journal of Hematology 2003;24(10):515-518
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo detect the IgH-MMSET fusion gene resulted from t (4;14) translocation in multiple myeloma and illuminate its significance.
METHODSIgH-MMSET fusion gene was detected in bone marrow specimens of 25 multiple myeloma (MM) patients and MM cell line NCI-H929 using reverse-transcription PCR (RT-PCR) assay followed by nested PCR to increase the sensitivity. The purified PCR products were cloned into pGEM-T vector and then sequenced using M13 forward primers. The fragment sequences were compared with that in GenBank to find matched sequences.
RESULTSOnly a 438 base pair long fragment was obtained after RT-PCR assay and was confirmed by sequencing to be a fusion gene product of IgH gene and MMSET gene in MM cell line NCI-H929. The breakpoints were located within the C micro region of IgH gene on chromosome 14 and intron 3 of MMSET gene on chromosome 4. IgH-MMSET hybrid transcripts were detected in 3 of 25 MM patients through nested PCR assay. The amplified fragments of the 3 patients were 237 base pairs (bp), 239 bp and 239 bp in length, respectively. The breakpoints on chromosome 4 were identical to that of NCI-H929 cell.
CONCLUSIONSThe formation of IgH-MMSET fusion gene is resulted from t (4;14) translocation in MM. The incidence rate is 12.0%. The presence of IgH-MMSET fusion gene may predict poor prognosis.
