Study on DNA methylation status of WT1 gene promoter in leukemia cell.
- Author:
Quan-shun WANG
1
;
Li YU
;
Yu ZHAO
;
Wei-dong HAN
;
Chun-ji GAO
;
Fang-ding LOU
Author Information
- Publication Type:Journal Article
- MeSH: Cell Line, Tumor; DNA Methylation; Genes, Wilms Tumor; Humans; Leukemia; genetics; Polymerase Chain Reaction; Promoter Regions, Genetic
- From: Chinese Journal of Hematology 2003;24(10):527-529
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo analyse the WT1 expression and its DNA methylation status of its promoter domain.
METHODThe expression of WT1 gene and its DNA methylation status were assayed in leukemia cell lines and normal peripheral blood mononuclear cells (PBMNC) by RT-PCR and MS-PCR.
RESULTSWT1 was overexpressed in HL60, K562 and KG1 leukemia cell lines, but not in U937 and PBMNC. Methylation of WT1 promoter was not observed in HL60 cells.
CONCLUSIONDNA methylation of WT1 gene promotor did not inhibit its expression. Other mechanisms may appear to regulate the WT1 expression.
