Analysis of caspases activity of hematopoietic progenitor cells and its significance in myelodysplastic syndromes.
- Author:
Jia-lin CHEN
1
;
Min XU
;
Xing-zhi SONG
;
Qi LI
;
Rong-sheng LI
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; Arsenicals; pharmacology; Caspase 3; Caspase 9; Caspases; metabolism; Hematopoietic Stem Cells; enzymology; physiology; Humans; Myelodysplastic Syndromes; enzymology; pathology; Oxides; pharmacology
- From: Chinese Journal of Hematology 2003;24(11):565-567
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the changes of apoptosis and the activity of caspases 3 and 9 in bone marrow hematopoietic progenitor cells in myelodysplastic syndromes (MDS).
METHODSBone marrow hematopoietic progenitor cells (including both CD(34)(+) and CD(34)(-) cells) were collected by negative selection in 34 patients with MDS. Apoptosis was measured with Annexin V assay and activities of caspases 3 and 9 by spectrophotometer.
RESULTS1. Apoptosis was significantly increased in MDS-RA (39.5%, P < 0.01) and MDS-RAEB (31.0%, P < 0.05), but was not different statistically in MDS-RAEBt/AML (18.8%) compared with that of control. 2. Activities of caspases 3 and 9 increased 45 and 20 fold in MDS-RA, increased 14 and 2 fold in MDS-RAEB, respectively and was not increased in MDS-RAEBt/AML compared with that of control. 3. Apoptosis and activities of caspases 3 and 9 reduced in 3 cases of MDS-RAEB group who progressed into AML.
CONCLUSIONIncreased activities of caspases 3 and 9 may be one of causes of excessive apoptosis in MDS. With progress to AML, activities of caspases 3 and 9 and apoptosis reduced. Reduced activity of caspase 9 may result in apoptosis "escape" and progression into AML.
