Effect of Tpo and/or IL-11 gene modified stromal cells on the expansion of CD34+ CD38- hematopoietic primitive progenitor cells.
- Author:
Ling-ling LU
1
;
Guang YANG
;
Liang LI
;
Xue-tao PEI
;
Ya-de ZHOU
;
Kai FENG
;
Ci-xian BAI
Author Information
- Publication Type:Journal Article
- MeSH: ADP-ribosyl Cyclase; analysis; ADP-ribosyl Cyclase 1; Antigens, CD; analysis; Antigens, CD34; analysis; Fetal Blood; cytology; Hematopoietic Stem Cells; physiology; Humans; Infant, Newborn; Interleukin-11; genetics; Membrane Glycoproteins; Stromal Cells; physiology; Thrombopoietin; genetics
- From:Chinese Journal of Hematology 2003;24(11):589-592
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of Tpo and/or IL-11 gene modified stromal cells on the expansion of CD(34)(+) hematopoietic stem/progenitor cells in cord blood.
METHODSRetroviral vectors containing Tpo or IL-11 gene were constructed and used to transfect the stromal cell line HFCL. Tpo and/or IL-11 mRNA was assayed by Northern blot. Non-modified stromal cells were used, CD(34)(+) hematopoietic stem/progenitor cells from cord blood were expanded on gene-modified stromal cells for 7 days. The phenotype of CD(34)(+)CD(38)(-) primitive progenitors was detected by flow cytometry.
RESULTSHFCL expressed Tpo and/or IL-11 mRNA after transfected by the retroviral vectors. The percentages of CD(34)(+)CD(38)(-) primitive progenitors in the cultures of Tpo, IL-11 and Tpo + IL-11 modified HFCL were (1.8 +/- 0.24)%, (1.62 +/- 0.23)%, and (2.45 +/- 0.28)%, respectively, which were higher than that in the control [(0.8 +/- 0.23)%].
CONCLUSIONThe stromal cells modified by Tpo and/or IL-11 gene were able to enhance ex vivo expansion of CD(34)(+) and CD(34)(+)CD(38)(-) hematopoietic stem/progenitor cells from cord blood.
