The role of vimentin during PRRSV infection of Marc-145 cells.
- Author:
Wei-Wei WANG
1
;
Lu ZHANG
;
Xiao-Chun MA
;
Ji-Ming GAO
;
Yi-Hong XIAO
;
En-Min ZHOU
Author Information
1. College of Animal Science and Veterinary Medicine, Shandong Agricultural University, TaiAn, Shandong 271018, China. wangww@163.com
- Publication Type:Journal Article
- MeSH:
Animals;
Antibodies;
immunology;
metabolism;
Cell Line;
Escherichia coli;
genetics;
metabolism;
Female;
Genetic Vectors;
genetics;
Mice;
Mice, Inbred BALB C;
Porcine Reproductive and Respiratory Syndrome;
genetics;
metabolism;
virology;
Porcine respiratory and reproductive syndrome virus;
physiology;
Protein Binding;
physiology;
Recombinant Proteins;
genetics;
immunology;
isolation & purification;
metabolism;
Swine;
Vimentin;
genetics;
immunology;
isolation & purification;
metabolism;
Viral Proteins;
metabolism
- From:
Chinese Journal of Virology
2011;27(5):456-461
- CountryChina
- Language:Chinese
-
Abstract:
The objective of this study was to investigate the function of vimentin in PRRSV infection. Vimentin gene from Marc-145 cells was amplified by RT-PCR, cloned into pET-28a vector and expressed in Escherichia coli BL21(DE3). The expressed vimentin was confirmed by Western blot and purified which was used to immunize BALB/c mice for the production of antibodies. Vimentin and antibodies were tested for blocking PRRSV infection of Marc-145 cells. The binding of vimentin to PRRSV N and GP5 proteins were tested by the ELISA. The results showed that vimentin gene was amplified successfully and expressed as identified by SDS-PAGE and Western blot. Mouse anti-vimentin antibodies were produced with the titer of 10(5). PRRSV infection of Marc-145 cells was blocked partially by vimentin while blocked completely by the antibobies. In addition, vimentin was bound N protein, but not GP5. These results provide additional information on PRRSV entry into Marc-145 cells.
