Expression of structural and non-structural proteins of severe fever with thrombocytopenia syndrome bunyavirus.
- Author:
Jing LU
1
;
Chuan LI
;
Fu-Shun ZHANG
;
Wei WU
;
Quan-Fu ZHANG
;
Li ZHANG
;
Tao WANG
;
Qin WANG
;
Pei-Hong QIU
;
Mi-Fang LIANG
;
De-Xin LI
Author Information
1. National Institute for Viral Disease Control and Prevention, China CDC, Beijing 102206, China.
- Publication Type:Journal Article
- MeSH:
Bunyaviridae Infections;
virology;
Cell Line, Transformed;
Fever;
virology;
HEK293 Cells;
Humans;
Orthobunyavirus;
genetics;
metabolism;
Thrombocytopenia;
virology;
Viral Nonstructural Proteins;
biosynthesis;
genetics;
Viral Structural Proteins;
biosynthesis;
genetics;
Virion;
genetics;
metabolism
- From:
Chinese Journal of Virology
2011;27(6):515-520
- CountryChina
- Language:Chinese
-
Abstract:
Severe fever with thrombocytopenia syndrome bunyavirus (SFTSV) is a novel phlebovirus, causing a life-threatening illness associated with the symptoms of severe fever and thrombocytopenia syndrome. The sequence and structure of the genome have already been illustrated in previous study. However, the characteristics and function of the structure and non-structure proteins is still unclear. In this study, we identified the density of the purified SFTSV virions as 1.135 g/mL in sucrose solution. Using RT-PCR method, we amplified the full coding sequence of RNA dependent RNA polymerase(RdRp), glycoprotein precursor (M), glycoprotein n (Gn), glycoprotein c (Gc), nuclear protein (NP) and non structural protein (NSs) of SFTSV (strain HB29). Respectively inserted the target genes into eukaryotic expression vector pcDNA5/FRT or VR1012, the target protein in 293T cell were successfully expressed. By analyzing the SFTSV virions in SDS-PAGE and using recombinant viral proteins with SFTS patients sera in Western blotting and Immunofluorescent assay, the molecule weight of structure and non-structure proteins of SFTSV were defined. The study provides the first step to understand the molecular characteristics of SFTSV.
