A GeXP based multiplex RT-PCR assay for simultaneous detection of twelve human respiratory viruses.
- Author:
Jin LI
1
;
Nai-Ying MAO
;
Meng QIN
;
Xiu-Mei HU
;
Meng-Jie YANG
;
Miao WANG
;
Chen ZHANG
;
Wen-Bo XU
;
Xue-Jun MA
Author Information
1. State Key Laboratory for Genetic Engineering and Molecular Virology, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
- Publication Type:Journal Article
- MeSH:
Humans;
Influenza A Virus, H1N1 Subtype;
genetics;
isolation & purification;
Orthomyxoviridae;
genetics;
isolation & purification;
Orthomyxoviridae Infections;
virology;
RNA Viruses;
genetics;
isolation & purification;
Real-Time Polymerase Chain Reaction;
instrumentation;
methods;
Respiratory Syncytial Viruses;
genetics;
isolation & purification;
Respiratory Tract Infections;
virology;
Reverse Transcriptase Polymerase Chain Reaction;
instrumentation;
methods;
Rhinovirus;
genetics;
isolation & purification;
Sensitivity and Specificity
- From:
Chinese Journal of Virology
2011;27(6):526-532
- CountryChina
- Language:Chinese
-
Abstract:
A GeXP based multiplex RT-PCR assay was developed to simultaneously detect twelve different respiratory viruses types/subtypes including influenza A virus, influenza B virus, influenza A virus sH1N1, parainfluenza virus type 1, parainfluenza virus type 2, parainfluenza virus type 3, human rhinovirus, human metapneumovirus, adenovirus, respiratory syncytial virus A, respiratory syncytial virus B and human bocavirus. Twelve sets of specific primers were designed based on the conserved sequences of available respiratory-virus sequence database. The specificity of the multiplex system was examined by positive specimens confirmed previously. The sensitivity to detect twelve respiratory viruses simultaneously was 10(3) copies/microL. Twenty four clinical specimens were further detected by this novel assay and the results were compared with that of the real-time RT-PCR. These results showed that this novel assay based on GeXP is a fast, sensitive, and high throughput test for the detection of respiratory virus infections.
