Effect of BYDV-MP nuclear localization signal on the movement of PVX.
- Author:
Ji-Jun YANG
1
;
Guo-Fu LIU
;
Yong-Mei SHEN
;
Xiao-Hui HUO
;
Xue-Song CAO
Author Information
1. College of Life Science, Liaocheng University, Liaocheng Shandong 252059, China.
- Publication Type:Journal Article
- MeSH:
Amino Acid Sequence;
Green Fluorescent Proteins;
genetics;
Luteovirus;
physiology;
Molecular Sequence Data;
Nuclear Localization Signals;
chemistry;
physiology;
Plant Viral Movement Proteins;
physiology;
Potexvirus;
genetics;
physiology
- From:
Chinese Journal of Virology
2012;28(1):35-44
- CountryChina
- Language:Chinese
-
Abstract:
Abstract:By using PVX derived vector pGR107, the effect of BYDV-MP nuclear localization signal on the movement of PVX was studied. BYDV-MP was cloned into pGR107 using GFP as an indicator. BYDV-MP was then shown to induce the systemic infection and exacerbate the symptom of PVX through infecting Nicotiana benthamiana. When the PVX gene encoding 25kD protein, which functioned as a systematic movemnet protein,was deleted and the above experiment was repeated, the result showed that BYDV-MP could compensate the systemic movement of PVX. A serial mutants with substitutions on the fifth, sixth and seventh amino acids of BYDV-MP nuclear localization signal was further constructed. It was found that the mutants at the fifth, sixth amino acids in BYDV-MP nuclear localization signal could only delay or weaken systemic movement of PVX whereas the mutant at seventh amino acid could entirely inhibit systemic movement of PVX.
