Prokaryotic expression and identification of HPV16 E7 protein.
- Author:
Yan-Yan JIA
1
,
2
;
Yue-Lan YIN
;
Chun-Guang BAI
;
Hong FU
;
Yun-Fei GAO
;
Zhi-Ming PAN
;
Xin-An JIAO
Author Information
1. Jiangsu Key Laboratory of Zoonosis
2. Ministry of Education, Key Lab for Avian Preventive Medicine, Yangzhou University, Yangzhou, 225009, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Enzyme-Linked Immunosorbent Assay;
Escherichia coli;
genetics;
Female;
Mice;
Mice, Inbred C57BL;
Papillomavirus E7 Proteins;
biosynthesis;
genetics;
isolation & purification;
Polymerase Chain Reaction;
Recombinant Proteins;
biosynthesis;
isolation & purification
- From:
Chinese Journal of Virology
2012;28(1):51-56
- CountryChina
- Language:Chinese
-
Abstract:
HPV16 E7 fusion protein was expressed in E. coli BL21, and its applied value for HPV was evaluated. HPV16 E7 gene was amplified by PCR, and cloned into prokaryotic expression vector pGEX6p-1. The recombinant plasmid was transformed into E. coli BL21, and HPV16 E7 fusion was expressed through IPTG induction. The expressed product was analyzed by SDS-PAGE and Western blot, subsequently purified according to Glutathione Sepharose 4B purification procedure. An indirect ELISA with the purified fusion protein as the coating antigen was then established to detect E7 serum antibodies from mice immunized with recombinant Listeria monocytogenes delivering HPV16 E7. The results demonstrated that the soluble fusion protein was highly expressed at 25 degrees C after induction with 0.5 mM IPTG. Furthermore, the result of Western blot analysis showed that the fusion protein had good specific reaction with an anti-E7 monoclonal antibody. Indirect ELISA result confirmed that the fusion protein could detect the serum antibodies against E7 with a titer of 1:200. The expressed GST-E7 fusion protein was immunocompetent, which was useful in the research of E7 biological function and therapeutic vaccine.
