Screening and identification of receptor reacting with nucleocapsid protein of duck enteritis virus.
- Author:
He HANG
1
;
Jun-Ting MAO
;
Ying YANG
;
Kai-Gong WANG
;
Bi-Jun ZHOU
;
Ming WEN
Author Information
1. College of Animal Science, Guizhou University, Guiyang, Guizhou 550025, China. hezhang514@yahoo.cn
- Publication Type:Journal Article
- MeSH:
Alphaherpesvirinae;
pathogenicity;
Animals;
Ducks;
virology;
Gene Library;
Liver;
virology;
Nucleocapsid Proteins;
genetics;
Plasmids;
Receptors, Virus;
analysis;
Two-Hybrid System Techniques
- From:
Chinese Journal of Virology
2012;28(1):63-66
- CountryChina
- Language:Chinese
-
Abstract:
To clarify the pathogenesis of Duck enteritis virus (DEV), the cDNA library of duck's liver infected by DEV and a bait plasmid containing DEV nucleocapsid protein (NP) gene were constructed, then the receptor was screened from the cDNA library plasmid by the yeast two-hybrid system and verified by GST pull-down test. The results showed that the capacity of the primary cDNA library was 1 x 106 CFU with insertion size from 0.5 to 1 kb, and the bait plasmid of pGBKT7-NP showed no self-activation. The receptor reacting with DEV NP in duck liver was initially confirmed as the protein kinase C inhibitor (PKCI). These results provide new clues for further investigation on pathogenesis of DEV.
