Genetic variation and pathogenicity analysis of highly pathogenic porcine reproductive and respiratory syndrome virus strain TJ in the course of attenuation.
- Author:
Leng-Xue
1
;
Zhen-Guang LI
;
Xi-Yu LI
;
Feng-Xue WANG
;
Xiu-Hua ZHANG
;
Wu-Hua
Author Information
1. Division of Zoonosis, Institute of Special Economic Animal and Plant Sciences, Chinese Academy of Agricultural Science, Jilin 132109, China. lengxue_79@163.com
- Publication Type:Journal Article
- MeSH:
Amino Acid Sequence;
Animals;
Genetic Variation;
Molecular Sequence Data;
Porcine Reproductive and Respiratory Syndrome;
virology;
Porcine respiratory and reproductive syndrome virus;
classification;
genetics;
isolation & purification;
pathogenicity;
Sequence Deletion;
Serial Passage;
Swine;
Vaccines, Attenuated;
genetics;
isolation & purification;
Virulence
- From:
Chinese Journal of Virology
2012;28(2):136-142
- CountryChina
- Language:Chinese
-
Abstract:
To develop an attenuated vaccine against the highly pathogenic porcine reproductive and respiratory syndrome (HP-PRRS) virus, the HP-PRRS virus strain TJ was attenuated by serial passages and plaque cloned every 5 to 10 passages in Marc-145 cells. Genetic variation and pathogenicity of HP-PRRSV strain TJ in the course of attenuation were analyzed. The results showed that the strain TJ sustained various sequence changes during the course of attenuation. Fifty-eight amino acids changes and a new continuous 120 amino acids deletion after the discontinuous 30 amino acids deletion (sites 481 and 533-561) occurred in strain TJ passages 140, and the position of 120 amino acids deletion was between 628 to 747 according to VR-2332. Animal test showed that the pathogenicity of strain TJ passages 20 was attenuated obviously, so we presume that genetic variation in nonstructural protein nsp2-nsp5, nsp7 and structural protein GP5 during the attenuation provides the molecular bases for the observed attenuated phenotype.
