Sequence comparison of the hemagglutinin gene of the duck-origin H9N2 subtype avian influenza viruses.
- Author:
Chun-He WAN
1
;
Guang-Hua FU
;
Long-Fei CHENG
;
Shao-Hua SHI
;
Hong-Mei CHEN
;
Chun-Xiang PENG
;
Fang LIN
;
Jian-Sheng LIN
;
Yu HUANG
Author Information
1. Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China. chunhewan@126.com
- Publication Type:Journal Article
- MeSH:
Animals;
China;
Ducks;
Hemagglutinin Glycoproteins, Influenza Virus;
chemistry;
genetics;
Influenza A Virus, H9N2 Subtype;
chemistry;
classification;
genetics;
isolation & purification;
Influenza A virus;
chemistry;
classification;
genetics;
Influenza in Birds;
virology;
Molecular Sequence Data;
Phylogeny;
Poultry Diseases;
virology;
Sequence Alignment
- From:
Chinese Journal of Virology
2012;28(2):158-164
- CountryChina
- Language:Chinese
-
Abstract:
To demonstrate the phylogenetic evolution, the molecular characteristics of the motif of HA protein cleavage site and the varieties at the receptor binding sites of the hemagglutinin gene of the duck-origin H9N2 subtype avian influenza viruses, sequence alignment and phylogenetic analysis were performed by MEGA 4.1 Neighbor-Joining method.. The results revealed that the duck-origin H9N2 AIV viruses originated from CK/BJ/1/94-like and North-Ame-like, all the duck-origin H9N2 AIV viruses from mainland China belonged to CK/BJ/1/94-like and formed multiple genotypes through complicated re-assortment, while other duck-origin H9N2 AIV, isolated from other countries in Aisa, American and European such as Korea, Japan, Alberta, Austria, Switzerland, Iran, belonged to the North-Ame-like phylogenetic lineage. The amino acids at positions 183, 190, and 226 of the receptor binding sites of North-Ame-like group isolates had highly conserved H, E and Q respectively. In contrast with duck-origin H9N2 AIV viruses isolates from mainland China, the amino acids had N at positions 183, A, T, or V at 190, L or Q at 226, which was the same as the chicken-origin H9N2 AIV from mainland China. Most newly isolated chicken-origin H9N2 AIV in Fujian Province in Southern China had L at position 226 emphasized the higher risk of cross-infection between the chicken-origin and duck-origin H9N2 AIV in China.
