The adjuvant effect of granulocyte macrophage colony stimulating factor (GM-CSF) in dengue virus and hepatitis C virus DNA vaccines.
- Author:
Jiang-Man WU
1
;
Hui CHEN
;
Zi-Yang SHENG
;
Juan WANG
;
Dong-Ying FAN
;
Na GAO
;
Jing AN
Author Information
1. Department of Microbiology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China. wujiangman2009@163.com
- Publication Type:Journal Article
- MeSH:
Adjuvants, Immunologic;
administration & dosage;
Animals;
Antibodies, Viral;
immunology;
DNA, Viral;
administration & dosage;
genetics;
immunology;
Dengue;
immunology;
prevention & control;
virology;
Dengue Vaccines;
administration & dosage;
genetics;
immunology;
Dengue Virus;
genetics;
immunology;
Female;
Granulocyte-Macrophage Colony-Stimulating Factor;
administration & dosage;
immunology;
Hepacivirus;
genetics;
immunology;
Hepatitis C;
immunology;
prevention & control;
virology;
Humans;
Immunization;
Mice;
Mice, Inbred BALB C;
Vaccines, DNA;
administration & dosage;
genetics;
immunology;
Viral Vaccines;
administration & dosage;
genetics;
immunology
- From:
Chinese Journal of Virology
2012;28(3):207-212
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the adjuvant effect of granulocyte macrophage colony stimulating factor (GM-CSF) in Flaviviridae virus DNA vaccines. After DNA immunization, the antibody levels of serum from mice were detected by ELISA and indirect immunofluorescence assay. Co-immunization of GM-CSF suppressed the immune responses induced by DV1 and DV2 candidate vaccines whereas enhanced the immune response induced by HCV C and E1 DNA vaccines. As genetic adjuvant for DNA vaccines, GM-CSF might display complex diversity on the immune responses: an augmentation or suppression due to different immunogens. Therefore, GM-CSF should be used with some cautions in clinic.
