Roles of N-glycosylation in immunity of prME and NS1 gene of JEV.
- Author:
Zi-Zhong ZHANG
1
;
Xue WANG
;
Jun-Jie ZAI
;
Le-Qiang SUN
;
Yun-Feng SONG
;
Huan-Chun CHEN
Author Information
1. Laboratory of Animal Infectious Diseases, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China. zzzhzyy@163.com
- Publication Type:Journal Article
- MeSH:
Animals;
Antibodies, Viral;
immunology;
Encephalitis Virus, Japanese;
genetics;
immunology;
metabolism;
Encephalitis, Japanese;
immunology;
virology;
Female;
Glycosylation;
Humans;
Mice;
Mice, Inbred BALB C;
Viral Nonstructural Proteins;
genetics;
immunology;
metabolism
- From:
Chinese Journal of Virology
2012;28(3):213-218
- CountryChina
- Language:Chinese
-
Abstract:
PrME and NS1 gene were the two main immuneprotect proteins of Japanese encephalitis virus (JEV), and they were also N-linked glycosylation proteins. To clear the effect of N-glycosylation on JEV immunity, the N-glycosylation site of prME and NS1 gene were eliminated by site-directed mutant PCR, subtituting the N to Q. And the the mutant genes were subcloned into eukaryotic expression plasmid. Four-weeks female mice were immuned with the wildtype and mutant gene by twice. The antibodies against prME were detected by ELISA and the neutralization antibodies were tested by viral neutralizing assay. The immunoprotection were determined by attack with JEV virulent strain. Compare with the wild-type gene immuned-groups, one N-glycan eliminated prME gene could induce a little higher ELISA antibody, neutralization antibody and immunoprotection, but the immunity of gene with both N-glycan absence was decreased. The similar status were observed in the wildtype and mutant NS1 groups. Thus these results show that the N-linked glycosylation in the prME and NS1 gene were correlated with the immunity, one glycan absent would enhance the immunity but both two loss would impair it.
