Establishment of minireplicon system for severe fever with thrombocytopenia syndrome bunyavirus.
- Author:
Xia-Li YU
1
;
Xiao-Lin JIANG
;
Tao WANG
;
Yu-Lan SUN
;
Shuo ZHANG
;
Chuan LI
;
Quan-Fu ZHANG
;
Mi-Fang LIANG
;
Zhen-Qiang BI
;
De-Xin LI
Author Information
1. National Institute for Viral Disease Control and Prevention, China CDC, Beijing 102206, China. yu_8605@sina.com
- Publication Type:Journal Article
- MeSH:
Bunyaviridae Infections;
virology;
Cloning, Molecular;
Genome, Viral;
Humans;
Phlebovirus;
genetics;
physiology;
Replicon;
Viral Proteins;
genetics;
metabolism;
Virus Replication
- From:
Chinese Journal of Virology
2012;28(3):246-251
- CountryChina
- Language:Chinese
-
Abstract:
Severe fever with thrombocytopenia syndrome bunyavirus is a newly emerging virus in China, enveloped with a tripartite, single-stranded RNA genome of negative polarity. The regulatory elements for viral transcription and replication, as well as encapsidation and packaging signals, are thought to be located within these noncoding regions (NCRs). The terminal nucleotides are genus specific and highly conserved. The function of the remaining nucleotides of the NCRs is still not well understood. In this study, we developed the plasmid-driven RNA polymerase I minireplicon system for SFTSV firstly, using reporter genes GFP and luciferase. The function of the noncoding regions of the three Bunyaviridae RNA segments (L, M, S) in transcription was analyzed. Reporter genes are successfully expressed in SFTSV minireplicon system. Our results suggest that the NCRs of SFTSV from all three segments contain the necessary signals to initiate transcription. Quantitative detection of the luciferase expression level shows that promoter activity in the three segments is different.
