Progress on development and research of coronavirus based vector.
- Author:
Yang YANG
1
;
Wen-Jie TAN
Author Information
1. National Institute for Viral Disease Control and Prevention, China CDC, Beijing 102206, China. yy860225@163.com
- Publication Type:Journal Article
- MeSH:
Animals;
Coronavirus;
genetics;
physiology;
Gene Expression;
Gene Transfer Techniques;
instrumentation;
Genetic Vectors;
genetics;
physiology;
Humans;
Virus Replication
- From:
Chinese Journal of Virology
2012;28(3):297-302
- CountryChina
- Language:Chinese
-
Abstract:
Due to the progress of targeted recombination and reverse genetics technique, it is possible to express foreign genes using coronaviruses as a vector via its unique transcription mechanism. Two types of coronavirus-based expression vectors have been developed; one is helper-dependent expression system and the other a single genome-based expression system. By modification of the infectious cDNA of the genome of coronaviruse, it has led to an efficient (> 50 microg/10(6)cells) and stable (> 30 passages) expression of the foreign gene. Moreover,several other features of coronavirus make it attractive as a vector. Firstly, the virulent coronaviruse can be converted into nonvirulent viruse by deleting the nonessential, group-specific genes. Secondly, the species and tissue tropism of coronaviruses can be manipulated by modification of their attachment and fusion protein S. Thus, coronavirus-based vector is becoming a promising and useful tool for vaccine development and gene delivery.
