Establishing a high-titer infectious avian influenza A (H5N1) pseudotyped viral particle.
- Author:
Xi-Jun LIU
1
;
Zhi-Ping GUO
;
Li-Ping SHEN
;
Yue WANG
;
Hai-Yan SHI
;
Guo-Hui ZHANG
;
Xun ZHANG
;
Sheng-Li BI
;
Hong-Lan ZHAO
Author Information
1. Institute of Medical Virology, School of Laboratory Medicine and Life Science, Wenzhou Medical College, Wenzhou 325035, China. liuxijun.jack@126.com
- Publication Type:Journal Article
- MeSH:
Animals;
Birds;
Cricetinae;
Genetic Engineering;
HEK293 Cells;
Hemagglutination;
Hemagglutinin Glycoproteins, Influenza Virus;
genetics;
Humans;
Influenza A Virus, H5N1 Subtype;
genetics;
physiology;
Influenza in Birds;
virology;
Neutralization Tests;
Transfection;
Viral Load;
genetics;
Virion;
genetics
- From:
Chinese Journal of Virology
2012;28(4):324-329
- CountryChina
- Language:Chinese
-
Abstract:
A transient four-plasmid cotransfection system was used to construct avian influenza A (H5N1) pseudotyped viral particle (H5N1Pp) by incorporating hemagglutinin (HA) protein and neuraminidase (NA) protein from H5N1 avian influenza virus onto Murine leukemia virus pseudotyped viral particles, the transmission electron microscopy, infectivity titer assay, hemagglutination assay, neutralization assay of H5N1Pp were studied. We established a pseudotyped H5N1 viral particle at a high titer of 10(8) Pp/mL, the morphology,the hemagglutination activity and neutralization specificity of H5N1Pp is simililar to wild H5N1 virus. The research result sets a platform for studying this virus, including its receptors, the functional analysis of HA and NA, neutralizing antibodies and anti-H5N1 drug development.
