Analyses on antigen epitopes and drug resistance mutations of HIV-1 gag and pol genes.
- Author:
Cheng-Zhang SHANG
1
;
Guo-Min CHEN
;
Huai-Yu ZHANG
;
Yi ZENG
Author Information
1. State Key Laboratory for Infections Disease Prevention and Control, Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention, Beijing 100052, China. cz_shang@163.com
- Publication Type:Journal Article
- MeSH:
Antigens, Viral;
immunology;
DNA Mutational Analysis;
Drug Resistance, Viral;
genetics;
Epitopes;
immunology;
HIV-1;
classification;
drug effects;
genetics;
immunology;
Human Immunodeficiency Virus Proteins;
genetics;
Mutation;
Phylogeny;
T-Lymphocytes, Cytotoxic;
immunology;
gag Gene Products, Human Immunodeficiency Virus;
genetics;
pol Gene Products, Human Immunodeficiency Virus;
genetics
- From:
Chinese Journal of Virology
2012;28(4):351-357
- CountryChina
- Language:Chinese
-
Abstract:
To study the CTL antigen epitopes and drug resistance mutations of HIV-1 gag and pol genes through analyzing gag and pol gene sequences. The HIV-1 gag and pol gene fragments were amplified using nested polymerase chain reaction. A total of 23 PCR sequences, 449 cloned gag sequences and 402 cloned pol sequences were obtained. Sequence analyses showed the 23 samples were subtype B or B'. A total of 4 in 8 CTL antigen epitopes appeared 8 mutations in consensus sequence of subtype B and B'. There were no mutations found in the PCR sequences, whereas a few mutations were found in clone sequences (9.80%) in 5 antigen epitopes in p24 region. Eighteen PIs-related mutations and 24 RTIs-related mutations were found in PCR sequences and clone sequences in pol gene region, in which 17 (94.44%) PIs-related mutations and 15 (62.50%) RTIs-related mutations were found only in the clone sequences, respectively. The results showed that the prevalence of HIV-1 drug resistance strains in this study was at a higher level (17.39%), suggesting that some samples were resistant.to existing antiviral drugs.
