The modified restriction of amino acids of HVR1, HVR2, HVR5, HVR7 in human adenovirus serotype 3 hexon.
- Author:
Zhi-Chao ZHOU
1
;
Huan-Xi LIANG
;
Ting LI
;
Tian-Hua ZHONG
;
Xing-Gui TIAN
;
Rong ZHOU
;
Xiao LI
Author Information
1. State Key Lab of Respiratory Disease, the First Affiliated Hospital of Guangzhou Medical College, Guangzhou Medical University, Guangzhou 510120, China.
- Publication Type:Journal Article
- MeSH:
Adenoviruses, Human;
genetics;
immunology;
Amino Acid Sequence;
Cell Line;
Computational Biology;
DNA, Recombinant;
genetics;
Genetic Engineering;
methods;
Genetic Vectors;
genetics;
Humans;
Models, Molecular;
Molecular Sequence Data;
Plasmids;
genetics;
Protein Conformation;
Species Specificity;
Viral Envelope Proteins;
chemistry;
genetics;
immunology
- From:
Chinese Journal of Virology
2012;28(4):372-381
- CountryChina
- Language:Chinese
-
Abstract:
The limitation of traditional Ad vectors result in wide application of capsid-incorporation of antigens into adenovirus capsid proteins, but usually it can't rescue virus successfully when we engineered the hypervariable regions (HVRs) of hexon in adenovirus serotype 3(Ad3) vector. So we deleted or retained some amino acids in HVR1, HVR2, HVR5, HVR7 predicted by bioinformatics, constructed recombinant Ad3 vector pBRAddeltaE3GFP-mHexon, and transfected it into AD293 cell to confirm the influence on the virus rescue. These data of amino acids that can be deleted or retained in the HVRs of Ad3 vector should provide operating foundation for antigen capsid-incorporation strategy in human adenovirus serotype 3, and also lay the groundwork for application of expressing foreign antigens in the hexon of human adenovirus serotype 3 as a platform of multivalent vaccine vectors.
