Degradation of 14-3-3beta appeared in apoptosis cell induced by PrP106-126 polypeptide.
- Author:
Peng SUN
1
;
Juan SONG
;
Jin ZHANG
;
Qin-Qin SONG
;
Xing GAN
;
Yu CUI
;
Chen GAO
;
Xiao-Zhen BO
;
Jun HAN
Author Information
1. Inner Mongolia Medical College, Hollyhock 010110, China.
- Publication Type:Journal Article
- MeSH:
14-3-3 Proteins;
metabolism;
Apoptosis;
drug effects;
HeLa Cells;
Humans;
Peptide Fragments;
pharmacology;
Prions;
pharmacology;
Proteolysis;
drug effects
- From:
Chinese Journal of Virology
2012;28(4):414-417
- CountryChina
- Language:Chinese
-
Abstract:
To investigate changes of 14-3-3beta from apoptosis induced by PrP106-126 polypeptide, HeLa cell was incubated with PrP106-126 for 4h or 8h. Nucleus changes and the expression of PARP were detected differently by Hoechst staining and Western blotting. Expressing of protein and mRNA from 14-3-3beta was determined by Western blotting and Real-time PCR. The results show that typical nucleus pyknosis and chip of apoptosis and degradation of PARP were induced by PrP106-126 peptide in HeLa cells. Degradation of 14-3-3beta appeared in apoptosis groups induced by PrP106-126 peptide. However, 14-3-3beta mRNA did not display any changes in apoptosis groups. This study indicated that degradation of antiapoptosis protein 143-3beta induced by PrP106-126 peptide may be one of pathogenesis mechanism of prion disease.
