Application of reversed-phase ion-pair chromatography for universal estimation of octanol-water partition coefficients of acid, basic and amphoteric drugs.
- Author:
Hui ZHU
1
;
Ri-Fang YANG
;
Liu-Hong YUN
;
Yu JIANG
;
Jin LI
Author Information
1. Beijing Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing 100850, China. zwhw797@gmail.com
- Publication Type:Journal Article
- MeSH:
1-Octanol;
chemistry;
Chromatography, Reverse-Phase;
methods;
Hydrogen-Ion Concentration;
Octanols;
chemistry;
Trifluoroacetic Acid;
Water;
chemistry
- From:
Acta Pharmaceutica Sinica
2009;44(9):1025-1028
- CountryChina
- Language:Chinese
-
Abstract:
This paper is to establish a reversed-phase ion-pair chromatography (RP-IPC) method for universal estimation of the octanol/water partition coefficients (logP) of a wide range of structurally diverse compounds including acidic, basic, neutral and amphoteric species. The retention factors corresponding to 100% water (logk(w)) were derived from the linear part of the logk'/phi relationship, using at least four isocratic logk' values containing different organic compositions. The logk(w) parameters obtained were close to the corresponding logP values obtained with the standard "shake flask" methods. The mean deviation for test drugs is 0.31. RP-IPC with trifluoroacetic acid as non classic ion-pair agents can be applicable to determine the logP values for a variety of drug-like molecules with increased accuracy.
