Theory of selectivity of RP-LC C18 column and its application.
- Author:
Wei-Qing ZHANG
1
;
Chang-Qin HU
Author Information
1. National Institute for the Control of Pharmaceutical and Biological Products, Beijing 100050, China.
- Publication Type:Journal Article
- MeSH:
Chromatography, High Pressure Liquid;
methods;
Chromatography, Reverse-Phase;
instrumentation;
methods;
Drug Contamination;
Pharmaceutical Preparations;
isolation & purification
- From:
Acta Pharmaceutica Sinica
2010;45(5):555-559
- CountryChina
- Language:Chinese
-
Abstract:
In recent years, more and more researchers focus on the selectivity of RP-HPLC columns. Among these columns, C18 column is the most commonly used the column. Yet even the same filler can not give us the same separation effect with the columns of different brands and different types, therefore, it is important to choose the suitable HPLC column for carrying out an experiment. After study in recent years, some column classification, selection methods (e.g.: column parameter method, F value method) have been achieved and contributed to the finding of suitable columns and reducing the blindness of column selection. This paper summarizes the advantages of these methods and their limitations in the application process, and gives some advice and expectations to pharmaceutical analysis works based on the works of other researchers.
