Effect of ketamine on transient outward potassium current of isolated human atrial myocytes.
- Author:
Su-juan KUANG
1
;
Chun-yu DENG
;
Xiao-hong LI
;
Xiao-ying LIU
;
Qiu-xiong LIN
;
Zhi-xin SHAN
;
Min YANG
;
Xi-yong YU
Author Information
1. Medical Research Center of Guangdong General Hospital, Guangdong Provincial Cardiovascular Institute, Guangdong Academy of Medical Sciences, Guangzhou 510080, China.
- Publication Type:Journal Article
- MeSH:
Adolescent;
Adult;
Aged;
Anesthetics, Dissociative;
administration & dosage;
pharmacology;
Dose-Response Relationship, Drug;
Female;
Heart Atria;
cytology;
Humans;
Ketamine;
administration & dosage;
pharmacology;
Male;
Middle Aged;
Myocytes, Cardiac;
cytology;
drug effects;
physiology;
Patch-Clamp Techniques;
Potassium Channels;
drug effects;
Young Adult
- From:
Acta Pharmaceutica Sinica
2010;45(7):849-852
- CountryChina
- Language:Chinese
-
Abstract:
The effects of ketamine on transient outward potassium current (I(to)) of isolated human atrial myocytes were investigated to understand the mechanism of part of its effects by whole-cell patch-clamp. Atrial myocytes were enzymatically isolated from specimens of human atrial appendage obtained from patients under going cardiac valve displacing. Ito is recorded in voltage-clamp modes using the patch-clamp technique at room temperature. Currents signals were recorded by an Axopatch 200B amplifier with the Digidata 1322A-pClamp 9.0 data acquisition system. Ketamine decreased I(to) of human atrial myocytes in a dose-dependent manner. The current-voltage curve was significantly lowered, 30, 100, 300, and 1000 micromol x L(-1) ketamine decreased respectively I(to) current density about (13.62 +/- 0.04)%, (38.92 +/- 0.05)%, (72.24 +/- 0.10)% and (83.84 +/- 0.05)% at the potential of 50 mV, with an IC50 of 121 micromol x L(-1). The I(to) activation curve, inactivation curve and the recovery curve were not altered by ketamine. So, ketamine concentration-dependently decreased I(to) of human atrial myocytes.
