Zinc ion implantation-deposition modification of titanium for enhanced adhesion of focal plaques of osteoblast-like cells.
- Author:
Juan XU
1
,
2
;
Min HU
;
Xin-Ying TAN
;
Chang-Kui LIU
Author Information
- Publication Type:Journal Article
- MeSH: Cell Adhesion; drug effects; Cell Line; Humans; Osteoblasts; cytology; drug effects; Photoelectron Spectroscopy; Titanium; pharmacology; Zinc Oxide; pharmacology
- From: Chinese Medical Journal 2013;126(18):3557-3560
- CountryChina
- Language:English
-
Abstract:
BACKGROUNDSurface modification by ion implantation-deposition is well established in materials science and can be an effective way to improve biocompatibility. The aim of this study is to evaluate the chemical composition of a modified titanium (Ti) surface after zinc (Zn) ion implantation and deposition and examine the effect of the modification on the formation of cellular focal adhesion plaques in vitro.
METHODScp-Ti discs were modified with Zn ion implantation and deposition via PIIID. The chemical composition of the surface modification was characterized by X-ray photoelectron spectroscopy (XPS). The formation of focal adhesion plaques on the modified Ti was investigated with human osteoblast-like MG-63 cells that were seeded onto the Ti surfaces and quantified by morphometric analysis under a confocal microscope.
RESULTSXPS data revealed that the modified Zn-Ti surface consisted of Ti, oxygen, Zn, and carbon. In addition, Gaussian fitting of the spectra indicated that the modified surface contained titanium dioxide and zinc oxide. After 6 hours of MG-63 cell culture, there were significantly more focal adhesion plaques on the modified surfaces than observed on the nonmodified Ti (P < 0.05).
CONCLUSIONZn ion implantation and deposition greatly improved the biocompatibility of Ti for the growth of MG-63 cells.