Influence of LY294002 on protein kinase B expression in salivary adenoid cystic carcinoma (SACC)-83 and SACC-LM cells.
- Author:
Ming-liang YANG
1
;
Lei SHEN
;
Chang-fu SUN
Author Information
- Publication Type:Journal Article
- MeSH: Carcinoma, Adenoid Cystic; drug therapy; enzymology; Chromones; pharmacology; Enzyme Inhibitors; pharmacology; Humans; Morpholines; pharmacology; Proto-Oncogene Proteins c-akt; drug effects; metabolism; Salivary Gland Neoplasms; drug therapy; enzymology; Tumor Cells, Cultured
- From: Chinese Journal of Stomatology 2006;41(6):358-359
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the influence of LY294002 on protein kinase B expression in salivary adenoid cystic carcinoma cells (SACC).
METHODSUsing Western-blot and RT-PCR detected the protein and mRNA level of protein kinase B (PKB) both in cell line, absent and present LY294002. The data were analysed by t-test.
RESULTSThe expression and transcription level of PKB (Ser(473)) in SACC-83 was lower than in SACC-LM (P < 0.05). The expression of PKB (Ser(473)) in SACC cell lines with LY294002 was lower than in SACC cell lines without LY294002 (P < 0.01). The transcription level of PKB showed no difference in SACC cell lines with and without LY294002 (P > 0.05).
CONCLUSIONSIn vitro, the protein expression and transcription level of PKB (Ser(473)) is higher in SACC-LM cell line than in SACC-83 cell line. In vitro, LY294002 can inhibit protein expression of PKB (Ser(473)) in SACC-83 cells and SACC-LM cells.
