Selection and quantitative detection of target genes in oral squamous cell carcinoma.

Li-qiong Duan; Wan-tao Chen; Ming-bin Zhang; Yong-jie HU; Yan LÜ; Ming Yan

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Selection and quantitative detection of target genes in oral squamous cell carcinoma.

Author: Li-qiong DUAN ^{1
,

2} ; Wan-tao CHEN ; Ming-bin ZHANG ; Yong-jie HU ; Yan LÜ ; Ming YAN
Author Information

1. Department of Oral and Maxillofacial Surgery, School of Stomatology
2. Ninth People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200011, China.
Publication Type:Journal Article
MeSH: Adult; Aged; Biomarkers, Tumor; genetics; Carcinoma, Squamous Cell; genetics; Female; Humans; Male; Middle Aged; Mouth Neoplasms; genetics; Oligonucleotide Array Sequence Analysis; methods; Reverse Transcriptase Polymerase Chain Reaction; methods
From: Chinese Journal of Stomatology 2006;41(8):456-460
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo select and identify the target genes related to oral squamous cell carcinoma (OSCC) and provide target genes for designing oligo-nucleotide functional microarray of OSCC.
METHODSGenes possibly related to oral squamous cell carcinoma were selected from the 5 years' published data of differently expressed profiles with microarray testing in OSCC. Then mRNA expression of selected genes were evaluated by real time quantitative polymerase chain reaction (RT-PCR) in 22 cases of OSCC, including tumor tissues and paried normal mucosas and quantified according to an internal control GAPDH.
RESULTSEight genes were tested. The overexpression of SPARC, PDGF-A, SERPINE1, TGF-beta(1) and VEGF-C genes were measured in 16, 18, 16, 20, 18 cases of tumor specimens, respectively. The expression of CK15 gene was lower than that of its normal tissue. There were overexpression of CCND1, BIRC3 in tumor tissues, but there was no significant difference of CCND1 and BIRC3 expression between tumor tissue and normal tissue (P > 0.05).
CONCLUSIONSSPARC, PDGF-A, SERPINE1, TGF-beta(1), VEGF-C and CK15 genes were closely related to tumor progress of OSCC. They can be used as the target genes for designing oligo-nucleotide functional microarray of OSCC.