Synthesis of a bio-active bone-matrix material and study of the cellular biocompatibility.
- Author:
Jie HAO
1
;
Qixin ZHENG
;
Xiaodong GUO
;
Daping QUAN
;
Binghong LUO
Author Information
1. Department of Orthopedics, Union Hospital, Tongji Medical College of Huazhong Science and Technology University, Wuhan 430022, China. hjie2005@yahoo.com.cn
- Publication Type:Journal Article
- MeSH:
Aspartic Acid;
chemistry;
Biocompatible Materials;
Bone Matrix;
cytology;
Lactic Acid;
chemistry;
Mesenchymal Stromal Cells;
cytology;
Polyesters;
Polyethylene Glycols;
chemistry;
Polyglycolic Acid;
chemistry;
Polymers;
chemistry;
Tissue Engineering
- From:
Journal of Biomedical Engineering
2005;22(3):433-437
- CountryChina
- Language:Chinese
-
Abstract:
To prepare poly(lactic acid/glycolic acid/ asparagic acid-co- polyethylene glycol) (PLGA-[ASP-PEG]) and examine the cellular biocompatibility. PLGA-[ASP-PEG] was obtained by bulk ring-opening copolymerization method, examined by infrared spectrometry (IR) and 1H nuclear magnetic resonance spectroscopy (1H NMR). Bone marrow stromal cells(BMSCs) were cultured with PLGA-[ASP-PEG] (experiment gruop) and PLGA (control group) in vitro respectively, and were observed by phase-contrast microscopy and scanning electron microscopy. The resuls showed that PLGA-[ASP-PEG] was obtained and proved by IR and 1H NMR. The BMSCs of the experiment group could well attach to and extend on the surface of the PLGA-[ASP-PEG], and could proliferate and secrete better extracellular matrix, compared with control. The PLGA-[ASP-PEG] has good cellular a biocompatibility. It can be used as a biomaterial for bone tissue engineering.
