Primary research of reconstruction of complete biological tissue engineered blood vessel.
- Author:
Yan ZHANG
1
;
Taihua LIU
;
Zonglai JIANG
;
Xiuhua ZHANG
;
Bo LIU
;
Yuquan LI
Author Information
1. Department of Human Anatomy, Second Military Medical University, Shanghai 200433, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Arteries;
Blood Vessel Prosthesis;
Cells, Cultured;
Coculture Techniques;
Dogs;
Microscopy, Electron, Transmission;
Muscle, Smooth, Vascular;
cytology;
Myocytes, Smooth Muscle;
cytology;
Swine;
Tissue Engineering;
Tissue Scaffolds
- From:
Journal of Biomedical Engineering
2005;22(3):448-451
- CountryChina
- Language:Chinese
-
Abstract:
To explore primarily the reconstruction of biological tissue engineered blood vessel in vitro, the decellularised scaffolds were obtained from swine common carotid arteries by enzyme digestion, and vascular smooth muscle cells (VSMCs) were isolated from canine thoracic aorta and subcultured and purified. The VSMCs were seeded into the inner surface of scaffolds and they were cocultured in vitro for 4 weeks. Histological staining and transmission electron microscopy were used to observe the growth of canine VSMCs on swine decellularized arterial scaffolds. The results showed the seeded VSMCs grew well on the decellularized scaffold throughout the duration of 4 weeks, which suggests that the method in this article is practicable for reconstructing complete biological tissue engineering blood vessel.
